Advantagen | Job | Note | Time | (£) |
|---|---|---|---|
| PCR Cloning | PCR cloning of a ~1.5 kb DNA in to a specific vector | 1.5 -2 wks | £400 |
| SMD 1 | Site-directed mutagenesis with 1-3 nt to change | 1.5-2 wks | £350 |
| SDM 2 | with more than 3 nt to change, gene deletion, insertion | 1.5-2 wks | £500 |
| Tagging | add epitope tags, eg, HA, myc, Flag-tag or GFP etc to a gene | 1.5-2 wks | £400 |
| RT-PCR | RT-PCR to clone cDNA from specific tissues | 2-3 wks | £2000 |
| siRNA | vector based siRNA construct | 2-3 wks | £350 |
| Sub-cloning 1 | DNA sub cloning using common enzymes | 1-2 wks | £200 |
| Sub-cloning 2 | Sub-cloning involves rare enzymes or incompatible ends | 1-2 wks | £300 |
| BAC/YAC | BAC, YAC DNA cloning | call |
|
| promoter | genomic DNA cloning, promoter cloning | call |
|
| Maxi-prep 1 | plasmid DNA maxi-prep by ultracentrifuge, 1 mg, 1st clone | 1 week | £100 |
| 2nd and more clones | £75 |
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| Maxi-prep 2 | plasmid Maxi-prep by column purification, 1 mg | 1 week | £60 |
| retro vector | DNA cloning in to retrovirus vector | 1-3 wks | £400 |
| Ad vector | DNA cloning into adenovirus vector | 2-4 wks | £600 |
| Genome | tail DNA genome typing by PCR | £6 |
|
| Library | cDNA library screen, yeast 2-hybrid library, phage display, etc | call |
|
Please also Contact us if the DNA Cloning work you want is not listed above. 20% discount for all new customers! |
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We also provide good valued super competent cells and DNA ladders.
